Antibody Affinity Testing
Service Features
Antibody affinity testing by SPR, BLI, and ELISA, measuring KD and association/dissociation rate constants, assessing antibody-antigen binding specificity, and supporting candidate screening and pair validation.
Service Overview
Antibody affinity testing supports antibody discovery, pair screening, and quality assessment. It measures the equilibrium dissociation constant KD and association and dissociation rate constants for antigen-antibody binding and compares binding across clones or lots.
SPR, BLI, and ELISA platforms are supported, with the method chosen based on molecular weight, sample purity, and buffer system; sensorgrams, fitted curves, and a test report are provided.
When sample volume is limited or buffer conditions are unusual, method development and pilot experiments can be performed first.
Service Advantages
- Multiple platforms: SPR, BLI, and ELISA.
- Complete KD and binding kinetics report.
- Parallel comparison and ranking of candidate clones.
- Method development and pilot experiments supported.
- Sensorgrams and raw data delivered together.
Service Workflow
Service workflow
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1
Requirements and sample evaluation
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2
Method selection and quote
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3
Pilot or formal testing
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4
Data analysis and report delivery
Routine samples are reported in 5-7 business days; complex systems depend on method development progress.
Customer Provides
Purified antibody or test sample with concentration information.
Antigen information (sequence, molecular weight, tag).
Buffer system and testing objective.
Ready to Start Your Project?
Our technical team can help with experimental design, sample evaluation and study execution. Contact us for a consultation or quote.
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