Nanobody Production
Service Features
Nanobodies (VHH) are selected via alpaca immunization and phage display. Their small size, deep tissue penetration, and high stability suit difficult targets, enzyme inhibition, and in vivo diagnostics.
Service Overview
Nanobodies are single-domain antibodies (VHH) derived from camelid heavy-chain antibodies, about 15 kDa, with strong tissue penetration, heat and pH tolerance, and easy recombinant expression.
The workflow includes alpaca immunization, library construction, phage display screening, sequence cloning, and recombinant expression, yielding sequence-defined VHH candidates.
Nanobodies suit difficult-to-express or highly conserved targets, enzyme inhibition, in vivo imaging, and diagnostic reagent development, and can be humanized or formatted into multivalent molecules.
Service Advantages
- Small size (about 15 kDa) with strong tissue penetration.
- Heat and pH tolerant for demanding environments.
- Efficient expression in E. coli, yeast, and other systems.
- Defined sequence facilitates humanization and multivalent engineering.
- Recognizes epitopes that conventional antibodies cannot easily access.
Service Workflow
Service workflow
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1
Antigen preparation and alpaca immunization
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2
Library construction and display screening
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3
Sequence cloning and expression
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4
Affinity testing and delivery
Project duration is about 10-14 weeks, depending on immune response and screening results.
Customer Provides
High-purity antigen and concentration.
Species and target information.
Application scenario and assay requirements.
Ready to Start Your Project?
Our technical team can help with experimental design, sample evaluation and study execution. Contact us for a consultation or quote.
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