Monoclonal Antibody Production
Service Features
Our antibody platform has extensive experience in mouse monoclonal antibody development, with a mature technical route and stable, sensitive products. We continuously optimize immunization, hybridoma fusion, and positive clone screening to significantly improve fusion and positive rates.
Service Overview
Compared with polyclonal antibodies, monoclonal antibodies target a single epitope with higher specificity and sensitivity, eliminating serological cross-reactivity between different cells and microbial species. They play important roles in cell surface marker research, soluble antigen purification, antibody structure and function studies, and antibody drug development. We provide a complete custom monoclonal antibody solution from antigen design and animal immunization to antibody purification and validation, with hybridoma and phage display platforms.
Service Advantages
- One-stop solution: from gene synthesis and antigen expression to antibody production.
- Scalable production: professional large-scale monoclonal antibody production for diagnostic-grade volumes.
- Strict QC: application-driven custom antibodies for ELISA, WB, IHC, and clinical platforms such as chemiluminescence, colloidal gold, and latex-enhanced turbidimetric immunoassay.
Service Workflow
Project analysis and design
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1
Plan discussion
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2
Contract signing
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3
Project initiation
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4
Antigen preparation
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5
Animal immunization
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6
Titer testing
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7
Screening and expansion
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8
Antibody purification
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9
Antibody validation
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10
Results delivery
The custom monoclonal antibody service follows an 11-step workflow to ensure accurate, reliable results.
Customer Provides
1. Recombinant protein: at least 2-3 mg, purity above 90%, concentration at least 0.5 mg/mL, molecular weight above 10 kDa.
2. Peptide: 1 mg naked peptide, 3-4 mg carrier-conjugated peptide (KLH/BSA/OVA), naked peptide purity above 90%, sequence length at least 10 amino acids.
3. For virus, bacteria, band, native protein, or other antigen types, please inquire; antigen preparation can also be commissioned to Proabbio.
Service Description
| Item | Description |
|---|---|
| Deliverables | 2-5 hybridoma cell lines and 5-10 mg of antibody purified from the best ascites. Validation report: mouse serum titer, hybridoma construction (fusion rate, positive rate), hybridoma cloning, culture supernatant antibody specificity, and ascites ELISA titer, with optional WB, IP, and IF validation. |
| Notes | Specific scope, sample requirements, and acceptance criteria are subject to the project plan confirmed by both parties. |
Ready to Start Your Project?
Our technical team can help with experimental design, sample evaluation and study execution. Contact us for a consultation or quote.
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