Rapid Polyclonal Antibody Production
Service Features
Centered on antibody technology, the rapid polyclonal antibody platform provides polyclonal antibody production in rabbits, mice, goats, and other species, with a turnaround of about 1-1.5 months.
Service Overview
Polyclonal antibody production immunizes a host animal to generate multiple antibody clones that recognize multiple epitopes and cause agglutination and precipitation. It is widely used in immunological diagnostics and often as WB enzyme-labeled secondary antibodies and ELISA coating antibodies. We provide polyclonal antibody production in rabbits, mice, goats, and other species.
Service Advantages
- Diversity: multiple antibody clones cover multiple antigen regions for broader recognition.
- High specificity: precise target antigen recognition with reduced non-specific binding.
- High affinity: strong binding to the target antigen.
- Flexibility: suitable for many applications.
- Robustness: other clones still work if some antibodies fail.
- Cost-effective: multiple antibody clones from one project.
Service Workflow
Project analysis and design
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1
Plan discussion
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2
Contract signing
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3
Project initiation
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4
Immunogen testing
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5
Animal immunization
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6
Titer testing
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7
Screening and expansion
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8
Antibody purification
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9
WB/IHC validation
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10
Results delivery
Rapid polyclonal antibody production takes about 1-1.5 months, depending on immune response and assay results.
Customer Provides
Recombinant protein: at least 2-3 mg, purity above 90%, concentration at least 0.5 mg/mL, molecular weight above 10 kDa.
Peptide: 1 mg naked peptide, 3-4 mg carrier-conjugated peptide (KLH/BSA/OVA), naked peptide purity above 90%, sequence length at least 10 amino acids.
For virus, bacteria, band, native protein, or other antigen types, please inquire; antigen preparation can also be commissioned to Proabbio.
Service Description
| Item | Description |
|---|---|
| Service content | Antigen QC, animal immunization, titer testing, blood collection, antibody purification, and WB / ELISA validation. |
| Species | Rabbit, mouse, rat, goat, etc. |
| Antibody type | Polyclonal antibody; serum or purified antibody. |
| Purification | Protein G affinity purification / antigen affinity purification. |
| Turnaround | About 1-1.5 months with customer-supplied qualified antigen; if antigen preparation or peptide synthesis is required, the timeline is assessed separately. |
| Deliverables | Purified antibody or serum, titer and purity reports, WB / ELISA validation results. |
| Customer provides | Antigen and quality information; or gene sequence, plasmid, peptide, small molecule, and other raw material information. |
| Notes | Specific antigen amount, antibody amount, purification method, and acceptance criteria are subject to the project plan confirmed by both parties. |
Ready to Start Your Project?
Our technical team can help with experimental design, sample evaluation and study execution. Contact us for a consultation or quote.
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