DNA Pull-Down Assay
Service Features
DNA pull-down studies DNA-protein interactions and gene regulation, revealing regulatory networks, signaling pathways, and disease mechanisms.
Service Overview
DNA pull-down studies protein-DNA interactions. Specific probes (commonly desthiobiotin-labeled) are designed against the regulatory region of the target gene and incubated with nuclear extract so DNA-binding proteins bind the target sequence. Streptavidin beads enrich the complex, and proteins are identified by WB or mass spectrometry.
Service Advantages
- High specificity: highly specific enrichment of proteins interacting with the target DNA sequence with low background.
- Quantitative: fluorescent or radioactive probes measure binding strength and affinity.
- High-throughput: multiple DNA-protein interactions can be studied at once.
- Low sample input: only small amounts are needed.
- Broad applicability: works in vitro and in vivo for gene regulation, DNA repair, chromatin structure and function, and more.
Service Workflow
Order and sample intake
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1
Sample preparation and processing
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2
Probe labeling
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3
Protein-probe co-incubation
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4
WB detection or mass spectrometry
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5
Results delivery
The DNA pull-down service follows a 6-step workflow to ensure accurate, reliable results.
Customer Provides
1. DNA sequence information or a DNA overexpression plasmid.
2. Cell samples.
3. For WB detection, a target-specific primary antibody.
4. Sample requirements:
Plasmid: at least 10 ug;
Frozen cells: 2 vials, or revived cells: 2 flasks;
Plant or animal tissue: at least 500 mg;
Cell pellet: at least 2x10^7 cells.
Note: revived cells can ship at ambient temperature; all other samples must ship on dry ice.
Service Description
| Item | Description |
|---|---|
| Deliverables | Recombinant plasmid containing the target gene, bacterial culture, and sequencing report. SDS-PAGE silver stain image of DNA pull-down proteins. WB report or mass spectrometry analysis report. |
| Notes | Specific scope, sample requirements, and acceptance criteria are subject to the project plan confirmed by both parties. |
Ready to Start Your Project?
Our technical team can help with experimental design, sample evaluation and study execution. Contact us for a consultation or quote.
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