Dual-Luciferase Reporter Assay
Service Features
Dual-luciferase assays offer high sensitivity, accurate internal calibration, and flexible applications, improving research efficiency and reducing experimental bias. They are a gold-standard method for mechanistic studies.
Service Overview
Dual-luciferase assays are a key method for transcriptional regulation studies, mainly validating interactions between promoters and transcription factors, or between miRNAs and target genes. Firefly luciferase serves as the experimental reporter and Renilla luciferase as the internal control; luminescence from substrate catalysis is measured on a luminometer, providing high sensitivity and a wide detection range.
Service Advantages
- High sensitivity: 1,000 times more sensitive than Western blot.
- No endogenous interference: reporters are not endogenously expressed in plants or mammals.
- Unaffected by intracellular components.
- Convenient and wide range: over 7 orders of magnitude.
Service Workflow
Sequence evaluation and target prediction
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1
Gene synthesis
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2
Vector construction
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3
Cell transfection
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4
Dual-luciferase assay
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5
Data analysis
The dual-luciferase service follows a 6-step workflow to ensure accurate, reliable results.
Customer Provides
Gene template, transcription factor, target gene, or microRNA information.
Experimental cells: 293T cells are used by default unless otherwise specified; no customer provision is needed.
Service Description
| Item | Description |
|---|---|
| Deliverables | Experimental process and a complete report, including raw data, images, and analysis results. |
| Notes | Specific scope, sample requirements, and acceptance criteria are subject to the project plan confirmed by both parties. |
Ready to Start Your Project?
Our technical team can help with experimental design, sample evaluation and study execution. Contact us for a consultation or quote.
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