EMSA (Gel Shift) Assay
Service Features
Professional EMSA services for high-precision detection of DNA binding proteins, RNA binding proteins, and specific nucleic acid-protein interactions.
Service Overview
EMSA detects protein-nucleic acid interactions with qualitative and quantitative analysis and is a classic method for transcription factor research. Common formats include validation EMSA (mutant probes confirm the binding site), competitive EMSA (cold probe competition rules out false positives), and supershift EMSA (antibody recognition forms a ternary complex to confirm specific binding).
Service Advantages
- Directly detects protein-nucleic acid interactions with qualitative and quantitative information.
- Studies specific, non-specific, and sequence-specific interactions.
- Technically straightforward and relatively easy to perform.
- Adaptable through various labeling and detection methods to different experimental needs.
Service Workflow
Order and sample intake
-
1
Assay design
-
2
Probe synthesis
-
3
EMSA gel preparation
-
4
EMSA reaction
-
5
Electrophoresis detection
-
6
Color development
-
7
Results delivery
The EMSA service follows an 8-step workflow to ensure accurate, reliable results.
Customer Provides
1. Cells for nuclear protein extraction: more than 10^7 cells, at least 400 mg animal tissue, or at least 2 g plant tissue.
2. Probe sequence.
3. If a specific antibody is used: at least 50 uL of antibody at more than 0.5 mg/mL.
Ready to Start Your Project?
Our technical team can help with experimental design, sample evaluation and study execution. Contact us for a consultation or quote.
Talk to a Scientist