GST Pull-Down Assay
Service Features
GST pull-down uses a tagged known protein as bait, bound to glutathione resin, to capture interacting proteins for WB validation.
Service Overview
GST pull-down uses a tagged known protein (most commonly GST-tagged) as bait bound to glutathione affinity resin. When the target protein solution passes through the column, the bait and its interacting complex are captured; after elution, protein gel electrophoresis analyzes the interaction or identifies target proteins.
Service Advantages
- Specific binding: GST fusion enables specific binding to the target protein for identifying interaction partners.
- High throughput: multiple protein interactions can be studied simultaneously.
- Simple and flexible: easy to perform with adjustable conditions.
- Direct detection: no additional labeling or modification required.
- Quantitative: binding analysis provides affinity and strength information.
Service Workflow
Order and sample intake
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1
Prokaryotic expression vector construction for bait and prey proteins
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2
Induced expression of bait and prey proteins
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3
Purification of bait and prey proteins
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4
Pull-down interaction analysis
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5
WB confirmation of interacting proteins
The GST pull-down service follows a 6-step workflow to ensure accurate, reliable results.
Customer Provides
Purified target and bait proteins (Proabbio can also construct them), or cell lysate supernatant containing the bait protein; primary antibody for detection (Proabbio can also provide it).
Samples should be stored frozen at -20C and shipped on ice packs.
Service Description
| Item | Description |
|---|---|
| Deliverables | Complete experimental report, SDS-PAGE and WB results. |
| Notes | Specific scope, sample requirements, and acceptance criteria are subject to the project plan confirmed by both parties. |
Ready to Start Your Project?
Our technical team can help with experimental design, sample evaluation and study execution. Contact us for a consultation or quote.
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