Luciferase Complementation (LCA) Assay
Service Features
Luciferase complementation (LCA) fuses luciferase fragments to two proteins of interest. When the proteins bind, luciferase activity is restored and catalyzes substrate luminescence, suitable for tobacco transient expression systems.
Service Overview
Luciferase complementation (LCA) determines whether two proteins interact. Two luciferase fragments are fused to the proteins of interest; if the proteins bind, luciferase activity is restored and catalyzes substrate luminescence. LCA in tobacco transient expression is simple, sensitive, reliable, efficient, and low-background, and scales to large-scale interaction screening and validation.
Service Advantages
- High sensitivity: detects picomolar protein interactions, suitable for low-abundance proteins.
- Real-time monitoring: fast luciferase reaction and strong signal allow monitoring without fixation or lysis.
- Non-destructive: performed in living cells without disrupting cell structure.
- Wide dynamic range: covers weak to strong interactions.
Service Workflow
Vector construction and co-transformation|Agroinfiltration of tobacco leaves|Luciferase activity assay|Data analysis and results delivery
Customer Provides
Genes synthesized by Proabbio:
Provide the protein CDS sequence.
Customer-provided template plasmid:
1. Customer-provided vector or template: a vector MCS sequencing report or template sequencing result is required; otherwise an additional sequencing fee applies.
2. Customer-provided bacterial culture: volume at least 0.5 mL; glycerol stocks older than one year must be revived before submission.
3. Customer-provided plasmid: at least 5 ug.
4. Bacterial cultures and liquid plasmids must ship on ice packs.
Service Description
| Item | Description |
|---|---|
| Deliverables | Return of remaining target gene plasmid. 3 negative controls and 1 experimental group by default. 3 leaf photographs. Final report and all raw data. |
| Notes | Specific scope, sample requirements, and acceptance criteria are subject to the project plan confirmed by both parties. |
Ready to Start Your Project?
Our technical team can help with experimental design, sample evaluation and study execution. Contact us for a consultation or quote.
Talk to a Scientist