Site-Directed Mutagenesis
Service Features
High-throughput site-directed mutagenesis introduces single- or multi-site mutations, deletions, insertions, and inversions into plasmid templates while preserving the original sequence.
Service Overview
Site-directed mutagenesis introduces base changes at specific sites in a DNA molecule to obtain a desired genotype and phenotype, widely used in disease models and stress-resistance breeding. High-fidelity GS polymerase ensures mutation accuracy; point mutations, insertions, or deletions can be introduced at any site, supporting DNA up to about 12 kb.
Service Advantages
- Accurate sequence: high-fidelity GS polymerase ensures accuracy and avoids unintended mutations.
- Any site: mutations can be introduced anywhere.
- Long fragments: point mutations, insertions, and deletions in DNA up to about 12 kb (including target gene and vector).
- One-stop solution covering template sequencing, gene synthesis, rapid cloning, vector construction, and protein expression and purification.
Service Workflow
Order intake
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1
Project evaluation
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2
Assay design
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3
Experiment
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4
QC
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5
Delivery
The site-directed mutagenesis service follows a 6-step workflow to ensure accurate, reliable results.
Customer Provides
1. Pre- and post-mutation gene sequences with mutation positions marked.
2. Vector information for the inserted target sequence, including sequence, resistance, sequencing primers, and cloning sites.
3. Samples:
Template plasmid containing the target sequence and resistance information;
Target vector (if it cannot be provided by Proabbio).
4. Sequencing chromatogram report of the template plasmid containing the target fragment.
Service Description
| Item | Description |
|---|---|
| Deliverables | About 4 ug of high-purity lyophilized plasmid DNA containing the target fragment. One tube of stab or glycerol stock containing the recombinant plasmid (TOP10 by default). Delivery documents: sequencing results, COA report, sequence alignment file, and .seq file. |
| Notes | Specific scope, sample requirements, and acceptance criteria are subject to the project plan confirmed by both parties. |
Ready to Start Your Project?
Our technical team can help with experimental design, sample evaluation and study execution. Contact us for a consultation or quote.
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