Subcloning & Vector Construction
Service Features
A range of high-throughput, low-cost cloning technologies directionally and seamlessly clone target genes into designated vector positions, independent of sequence and restriction sites.
Service Overview
Subcloning and vector construction are common genetic engineering techniques for inserting a target gene into vector DNA to build recombinant molecules. Subcloning typically transfers a DNA fragment to another vector using restriction enzymes, while vector construction inserts the target gene into an existing vector via PCR amplification and digestion. Our high-throughput, low-cost vector construction overcomes sequence and restriction-site constraints to achieve directional, seamless cloning.
Service Advantages
- Proprietary high-throughput, low-cost vector construction rapidly and efficiently builds various target vectors, significantly saving time.
Service Workflow
Order intake
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1
Project evaluation
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2
Assay design
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3
Cloning
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4
QC
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5
Delivery
The subcloning and vector construction service follows a 6-step workflow to ensure accurate, reliable results.
Customer Provides
1. Target sequence information.
2. Vector information for inserting the target sequence, including sequence, resistance, sequencing primers, and cloning sites.
3. Samples:
Template plasmid containing the target sequence and resistance;
Target vector (if it cannot be provided by Proabbio).
4. Sequencing chromatogram report of the target fragment in the template plasmid.
Service Description
| Item | Description |
|---|---|
| Deliverables | About 4 ug of high-purity lyophilized plasmid DNA containing the target fragment. One tube of stab or glycerol stock containing the recombinant plasmid (TOP10 by default). Delivery documents: sequencing results, COA report, sequence alignment file, and .seq file. |
| Notes | Specific scope, sample requirements, and acceptance criteria are subject to the project plan confirmed by both parties. |
Ready to Start Your Project?
Our technical team can help with experimental design, sample evaluation and study execution. Contact us for a consultation or quote.
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