Yeast One-Hybrid (Y1H) Assay
Service Features
Yeast one-hybrid assays study nucleic acid-protein interactions, identify DNA binding sites, discover potential binding proteins, and analyze DNA binding domain information.
Service Overview
Yeast one-hybrid (Y1H), derived from yeast two-hybrid, studies nucleic acid-protein interactions. It identifies DNA binding sites, discovers potential DNA-binding protein genes, and analyzes DNA binding domain information. The technique can study multiple protein interactions simultaneously, and reporter-gene validation plus dual screening effectively reduces false positives from non-specific interactions.
Service Advantages
- One-stop assisted testing with convenient, reliable conclusions.
- Years of library construction experience and four optimized total RNA extraction protocols that preserve RNA purity, integrity, and sample diversity.
- Simultaneous study of multiple protein interactions for high-throughput screening and analysis.
- Multi-step screening (reporter validation, dual selection) reduces false positives from non-specific interactions.
Service Workflow
Y1H library screening
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1
Order and sample intake
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2
Bait vector and bait strain construction
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3
Autoactivation testing
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4
Library screening
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5
Positive clone identification
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6
Results delivery
Yeast hybrid library construction
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1
Total RNA extraction
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2
mRNA isolation and purification
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3
Double-stranded cDNA synthesis and purification
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4
ds-cDNA and vector fusion
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5
E. coli library culture
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6
Library plasmid
Yeast hybrid validation|Bait and prey recombinant plasmid construction|Bait autoactivation and toxicity testing|Yeast co-transformation validation|Gradient spot assay validation
Customer Provides
1. Species information and sample type.
2. Gene sequence information.
3. Experimental objective.
Ready to Start Your Project?
Our technical team can help with experimental design, sample evaluation and study execution. Contact us for a consultation or quote.
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