Yeast Two-Hybrid
Service Features
We provide yeast hybrid library construction, nuclear-system yeast two-hybrid screening, membrane-system yeast two-hybrid screening, and yeast one/two-hybrid validation services.
Service Overview
Yeast two-hybrid identifies and detects protein-protein interactions with high sensitivity and broad applicability. Nuclear yeast two-hybrid, based on the GAL4 transcription factor, reflects protein interactions in living cells; membrane yeast two-hybrid uses a split-ubiquitin system that divides ubiquitin into N- and C-terminal halves, with ubiquitin-specific protease recognizing reconstituted ubiquitin to screen membrane protein interactions.
Service Advantages
- One-stop assisted testing with convenient, reliable conclusions.
- Years of library construction experience and four optimized total RNA extraction protocols ensure RNA purity and integrity.
- Directly detects protein-protein interactions without relying on other molecules or cells.
- Simultaneously screens and identifies multiple interactions for high-throughput analysis.
- Multi-step screening (reporter validation, dual yeast strain selection) reduces false positives.
- Quantifies interaction strength through reporter expression to further understand kinetics and function.
Service Workflow
Yeast hybrid library construction workflow:
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1
Order and sample intake
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2
Total RNA extraction and quality assessment
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3
mRNA isolation and purification
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4
Double-stranded cDNA synthesis and purification
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5
ds-cDNA and vector fusion
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6
Library quality validation and delivery
Yeast two-hybrid screening workflow:
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1
Bait plasmid construction and validation
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2
Bait autoactivation and toxicity testing
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3
Co-transformation of bait and library into yeast
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4
Positive clone selection
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5
Plasmid recovery and sequencing
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6
Interaction validation and results delivery
Strict workflows and stage-specific QC ensure accurate, reliable interaction identification.
Ready to Start Your Project?
Our technical team can help with experimental design, sample evaluation and study execution. Contact us for a consultation or quote.
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